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RESEARCH PRODUCT

Gelchromatographische refraktionierung, 1. Ein verfahren zur korrektur von gelchromatogrammen

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description

A mathematical method is proposed for calculating the gel chromatogram w(v0) after correction with the instrument spreading function D(v, v0) and for calculating D(v,v0) using experimental data on the basis, that a gel chromatographic polymer fraction of the chromatogram e(v) is collected and injected again. The chromatogram of this fraction c(v) can be described by the integral equation: where v0 is the elution volume and v1 is the volume at which the fraction is collected. The solution of this equation simultaneously with Tung's equation by way of minimizing is suggested.