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RESEARCH PRODUCT
Salmonella-reactive Synovial Fluid T-cell Clones in a Patient with Post-infectious Salmonella Arthritis
Thomas PorallaElisabeth HermannWerner-johannes MayetK H Meyer Zum BüschenfeldeBernhard Fleischersubject
AdultMaleSalmonella typhimuriumSalmonellaT-LymphocytesT cellImmunologyArthritismedicine.disease_causeCampylobacter jejuniPeripheral blood mononuclear cellMicrobiologyRheumatologyAntigenSalmonellaSynovitisSynovial FluidmedicineHumansImmunology and AllergySynovial fluidBlood CellsbiologyArthritisGeneral Medicinebiology.organism_classificationmedicine.diseaseVirologyClone Cellsmedicine.anatomical_structureSalmonella InfectionsCell Divisiondescription
From a patient with reactive arthritis following Salmonella typhimurium enteritis, synovial fluid T-lymphocytes were cloned and expanded in vitro. Seven out of 74 clones showed a marked proliferative response to antigens of heat-killed Salmonella typhimurium with autologous T-cell-depleted peripheral blood mononuclear cells as antigen-presenting cells. The Salmonella-reactive clones were of the CD4+ phenotype, antigen-induced proliferation could be inhibited by a monoclonal antibody to HLA class II. One clone recognized both Salmonella and Campylobacter jejuni antigens in the proliferation assay. The multiclonality of Salmonella-reactive synovial fluid T-cells indicates that the microorganisms have been present, at least transiently, within the affected joint and thus recruited specific T-lymphocytes that might initiate synovitis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1990-01-01 | Scandinavian Journal of Rheumatology |