6533b85cfe1ef96bd12bd51b

RESEARCH PRODUCT

Target Analysis and Retrospective Screening of Multiple Mycotoxins in Pet Food Using UHPLC-Q-Orbitrap HRMS

Yelko Rodríguez-carrascoLuigi CastaldoJosefa TolosaAlberto RitieniLuana IzzoAnna GaspariGiulia Graziani

subject

FusariumAnalyteHealth Toxicology and Mutagenesislcsh:MedicineTarget analysisFood ContaminationToxicologyMass spectrometryOrbitrap01 natural sciencesMass SpectrometryArticlelaw.inventionPet foodchemistry.chemical_compound0404 agricultural biotechnologyDogslawmycotoxinsco-occurrenceretrospective screeningAnimalsMycotoxinChromatography High Pressure LiquidMycotoxinChromatographybiologyChemistry010401 analytical chemistrylcsh:Rpet foodfood and beveragesHRMS-orbitrap04 agricultural and veterinary sciencesPetsbiology.organism_classification040401 food scienceAnimal Feed0104 chemical sciencesmonitoringCatsFusaric acid

description

A comprehensive strategy combining a quantitative method for 28 mycotoxins and a post-target screening for other 245 fungal and bacterial metabolites in dry pet food samples were developed using an acetonitrile-based extraction and an ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) method. The proposed method showed satisfactory validation results according to Commission Decision 2002/657/EC. Average recoveries from 72 to 108% were obtained for all studied mycotoxins, and the intra-/inter-day precision were below 9 and 14%, respectively. Results showed mycotoxin contamination in 99% of pet food samples (n = 89) at concentrations of up to hundreds &micro

yearjournalcountryeditionlanguage
2019-07-24Toxins
10.3390/toxins11080434http://dx.doi.org/10.3390/toxins11080434