6533b85cfe1ef96bd12bd5d2
RESEARCH PRODUCT
Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA
Alexandr MotorinYuri MotorinIsabelle Behm-ansmantMark Helmsubject
chemistry.chemical_classification0303 health scienceslcsh:QH426-470030302 biochemistry & molecular biologyRNA[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biologyReview ArticleBiologyMass spectrometryBioinformaticsBiochemistryReverse transcriptasePrimer extensionlcsh:Biochemistry03 medical and health scienceslcsh:GeneticsEnzymechemistryBiochemistryReagentReactivity (chemistry)Nucleotidelcsh:QD415-436Molecular Biology030304 developmental biologydescription
International audience; Naturally occurring cellular RNAs contain an impressive number of chemically distinct modified residues which appear posttranscriptionally, as a result of specific action of the corresponding RNA modification enzymes. Over 100 different chemical modifications have been identified and characterized up to now. Identification of the chemical nature and exact position of these modifications is typically based on 2D-TLC analysis of nucleotide digests, on HPLC coupled with mass spectrometry, or on the use of primer extension by reverse transcriptase. However, many modified nucleotides are silent in reverse transcription, since the presence of additional chemical groups frequently does not change base-pairing properties. In this paper, we give a summary of various chemical approaches exploiting the specific reactivity of modified nucleotides in RNA for their detection.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-04-01 | Journal of Nucleic Acids |