Polifenoliem bagātu ekstraktu iegūšana no Vaccinium ģints ogu spiedpaliekām, to izpēte un bioloģiskās aktivitātes raksturošana

6533b85dfe1ef96bd12bd7fe

RESEARCH PRODUCT

Polifenoliem bagātu ekstraktu iegūšana no Vaccinium ģints ogu spiedpaliekām, to izpēte un bioloģiskās aktivitātes raksturošana

Linards Kļaviņš

subject

ekstrakcija polifenoli antimikrobiālā aktivitāte brīvo radikāļu saistīšana Bioloģija Vaccinium

description

Latvijas teritorijā savvaļā sastopamās un kultivētās Vaccinium ģints ogas ir nozīmīgs resurss ar plašu, bet neapzinātu izmantošanas potenciālu, to sekundāro metabolītu sastāva izpēte ir nozīmīgs uzdevums, kas var iezīmēt šo ogu izmantošanas iespējas pārtikas rūpniecībā un medicīnā. Maģistra darba mērķis ir pētīt Vaccinium ģints ogu spiedpaliekās esošo polifenolu iegūšanu un to attīrīšanas iespējas, kā arī veikt to ķīmiskā sastāva izpēti un bioloģiskās aktivitātes raksturošanu. Veiktā ogu polifenolu ekstrakcijas apstākļu izpēte un optimizācija ļāva identificēt ultraskaņas ekstrakciju kā visefektīvāko ekstrakcijas metodi. Izmantojot virsmas reakcijas metodi, tika noskaidrota optimālā polifenolu un antociānu ekstrakcijā izmantojamā šķīdinātāja koncentrācija- ar trifluoretiķskābi paskābināts metanols vai etanols. Pierādīta 37 polifenolu klātbūtne iegūtajos ekstraktos un noteikti to daudzumi, kas ļāva veikt hemotaksonomijas analīzi. Vaccinium ģints ogu polifenolu koncentrātus (polifenolu saturs līdz 56%) raksturo augsta brīvo radikāļu saistīšanas spēja, kas tika noskaidrota veicot šūnu citometrijas testus, tāpat tika parādīta iegūto ekstraktu spēja kavēt dermas fibroblastu proliferāciju. Iegūtie ekstrakti parāda antimikrobiālu aktivitāti pret dažādām cilvēkam patogēnām baktērijām (Enterococcus, Streptococcus, Staphylococcus).

year	journal	country	edition	language
2017-01-01

https://dspace.lu.lv/dspace/handle/7/35164

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