6533b85dfe1ef96bd12bdbea

RESEARCH PRODUCT

Development a mitigation strategy of enniatins in pasta under home-cooking conditions

Emilia FerrerGuillermina FontJordi MañesA.b. Serrano

subject

ChromatographyChemistryExtraction (chemistry)food and beverages04 agricultural and veterinary sciencesMass spectrometryTandem mass spectrometry040401 food sciencechemistry.chemical_compound0404 agricultural biotechnologyBoilingTetrachlorideMycotoxinVolume concentrationEnniatin AFood Science

description

The effect of pasta cooking on the enniatins (ENs) contents was evaluated in white and whole-grain pasta. The cooking of pasta was performed for 10 min in boiling water. ENs were extracted from raw and cooked pasta by Solid-Liquid extraction with acetonitrile, and from cooking water by Dispersive Liquid–Liquid Micro-Extraction (DLLME) with a mixture of acetonitrile and carbone tetrachloride. Mycotoxin analysis was performed by Liquid Chromatography coupled to Tandem Mass Spectrometry. The effect of pasta cooking has a marked influence on the reduction of ENs levels. Reductions of 98–100% for ENA, 94–95% for ENA1, 14–49% for ENB and 53–65% for ENB1 were achieved after the process. ENs were detected at low concentration levels in water samples. Moreover, it was developed a mitigation strategy to reduce the ENs contents by the combining effect of the thermal treatment and the pH during cooking pasta. The modification of the pH in the cooking water to pH 4 resulted in percentages of reduction close to 100%, while the modification to pH 9 provided percentages from 44 to 100%. Two degradation products were tentatively elucidated after the thermal treatment by Liquid Chromatography-Quadrupole-Linear Ion Trap-Mass Spectrometry.

yearjournalcountryeditionlanguage
2016-01-01LWT - Food Science and Technology
https://doi.org/10.1016/j.lwt.2015.09.036