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RESEARCH PRODUCT
Augmented Passive Transfer of Contact Sensitivity in Severe Combined Immunodeficiency Mice and Its Dependence of Vβ8<sup>+</sup> Cells in the Picryl System
Alfredo SalernoM. MatteiFrancesco DieliGeoffrey L. AshersonA. BellaviaM.c. CaroleoV. Colizzisubject
Adoptive cell transferSevere combined immunodeficiencyRatónbusiness.industryImmunologyGeneral MedicineT lymphocytemedicine.diseasePicryl chloridechemistry.chemical_compoundImmune systemLymphatic systemchemistryImmunologyImmunology and AllergySyngenicMedicinebusinessdescription
The passive transfer of contact sensitivity using picryl chloride immune cells from H-2 syngenic BALB/c donors was analyzed in severe combined immunodeficiency (SCID) mice which lack functional T and B lymphocytes. H-2-restricted and antigen-specific contact sensitivity was transferred to SCID mice, and comparison between the level of contact sensitivity and the number of transferred cells showed a significantly more efficient transfer to SCID than to BALB/c mice. The cells passively transferring contact sensitivity were shown to carry the Vβ8 phenotype. Moreover, chromium-labeled cells from BALB/c PC1-primed donors localize normally in peripheral lymphoid organs, and an increased percentage of cell arrival in the ears is clearly observed in SCID after challenge with picryl chloride.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-01-01 | International Archives of Allergy and Immunology |