6533b85dfe1ef96bd12bdec1

RESEARCH PRODUCT

Upregulation of the netrin receptor (DCC) gene during activation of b lymphocytes and modulation by interleukins.

Françoise RoussetEric GarciaPascale CornilletJean-raymond TeyssierAline Laubriet

subject

AdultDeleted in Colorectal CancerTranscription GeneticT-LymphocytesPalatine TonsilBiophysicsReceptors Cell SurfaceBiologyLymphocyte ActivationBiochemistryCell LineNetrin Receptor DCCDownregulation and upregulationNetrinmedicineTumor Cells CulturedHumansRNA MessengerReceptorMolecular BiologyB cellB-LymphocytesReverse Transcriptase Polymerase Chain ReactionInterleukinsTumor Suppressor ProteinsfungiBrainCell BiologyDCC ReceptorMolecular biologyInterleukin-10Up-Regulationmedicine.anatomical_structureGenes DCCCell cultureImmunoglobulin superfamilyInterleukin-2Netrin ReceptorsCell Adhesion MoleculesImmunologic MemoryMuromonab-CD3

description

The DCC (deleted in colon cancer) gene has a brain restricted high expression pattern. It encodes a transmembrane protein of the immunoglobulin superfamily identified as the netrin-1 receptor. It might be a member of the so called "brain-lymphoid" molecules, which control key cell surface events. To test this hypothesis we have assessed the DCC mRNA level in human normal and malignant myeloid and lymphoid cells. A high mRNA content has been observed only in mature B cells at the secreting or presecreting stage. Expression of DCC was also assessed in the anti-CD40 model of immunopoiesis. Activation of purified tonsillar B cells by anti-CD 40 antibody strongly increased the DCC mRNA level and this effect was dramatically enhanced by the association of IL-2 + IL-10, which is a potent and selective in vitro inducer of the B cell memory phenotype. In contrast no effect has been detected after activation of T cells by anti-CD3. These data suggest that the DCC encoded netrin receptor is involved in B cell immunopoiesis.

yearjournalcountryeditionlanguage
2001-05-01Biochemical and biophysical research communications
10.1006/bbrc.2001.4902https://pubmed.ncbi.nlm.nih.gov/11355876