6533b85dfe1ef96bd12be6da

RESEARCH PRODUCT

Relationship between Auxin-Dependent Proton Extrusion and Plasmalemma Redox System in Maize Coleoptile Segments

Julio SalgueroS. Del Valle-tasconFrancisco Gonzalez-daros

subject

chemistry.chemical_classificationchemistry.chemical_compoundColeoptileBiochemistryProtonChemistryAuxinPhase (matter)KineticsBiophysicsExtrusionFerricyanideRedox

description

Abraded coleoptile segments reduce ferricyanide utilizing endogenous substrates. The kinetics of ferricyanide reduction shows an initial rapid rate followed by a slow rate of reduction. The duration of the initial phase is about 5 minutes. The slow phase is observed for more than 30 minutes. A Lineweaver-Burk plot of the slow phase was linear and revealed maximum velocity of the reaction of 42 nmols ferricyanide reduced/hour*coleoptile segment and an apparent Km (ferri) of 2.25mM.

yearjournalcountryeditionlanguage
1988-01-01
https://doi.org/10.1007/978-1-4684-8029-0_57