6533b85dfe1ef96bd12be84b

RESEARCH PRODUCT

Expression of metabotropic glutamate receptors in murine thymocytes and thymic stromal cells

Giuseppe BattagliaMarianna StortoIsabella ScrepantiUgo De GraziaLuigi FratiMaria Pia FelliFerdinando NicolettiAntonella CalogeroMarella MaroderGiuseppe RagonaAlberto Gulino

subject

CD4-Positive T-LymphocytesMalemedicine.medical_specialtyStromal cellNeuroimmunomodulationReceptor expressionBlotting WesternImmunologyGene ExpressionThymus GlandCD8-Positive T-LymphocytesReceptors Metabotropic GlutamateCell LineMicePhosphatidylinositol PhosphatesInternal medicineCyclic AMPmedicineAnimalsImmunology and AllergyCycloleucineRNA MessengerReceptorReverse Transcriptase Polymerase Chain ReactionChemistryMetabotropic glutamate receptor 5HydrolysisMetabotropic glutamate receptor 6Flow CytometryCell biologyMice Inbred C57BLNeuroprotective AgentsEndocrinologyMetabotropic receptormetabotropic glutamate receptors; tc-1s cells; thymocytesNeurologyMetabotropic glutamate receptorMetabotropic glutamate receptor 1Neurology (clinical)Stromal CellsSignal Transduction

description

RT-PCR combined with immunoblotting showed the expression of group-I (mGlu1 and 5) and group-II (mGlu2 and 3) metabotropic glutamate receptors in whole mouse thymus, isolated thymocytes and TC-1S thymic stromal cell line. Cytofluorimetric analysis showed that mGlu-5 receptors were absent in CD4(-)/CD8(-) but present in more mature CD4(+) CD8(+) and CD4(+)CD8(-) thymocytes. mGlu-1a receptors showed an opposite pattern of expression with respect to mGlu5, whereas mGlu2/3 receptor expression did not differ between double negative and double positive cells. mGlu receptors expressed in both thymic cell components were functional, as indicated by measurements of polyphosphoinositide hydrolysis or cAMP formation. These data suggest a possible role for mGlu receptor signalling in the thymus.

yearjournalcountryeditionlanguage
2000-09-21Journal of Neuroimmunology
https://doi.org/10.1016/s0165-5728(00)00269-1