6533b85dfe1ef96bd12be855

RESEARCH PRODUCT

Assessment of ISO Method 15216 to Quantify Hepatitis E Virus in Bottled Water

Antonio J. Martínez-murciaAlba Pérez-cataluñaEnric Cuevas-ferrandoWalter RandazzoWalter RandazzoGloria Sánchez

subject

0301 basic medicineMicrobiology (medical)viruses010501 environmental sciencesmedicine.disease_cause01 natural sciencesMicrobiologyArticleVirus03 medical and health sciencesConcentration methodsHepatitis E virusconcentration methodVirologymedicinelcsh:QH301-705.50105 earth and related environmental sciencesDetection limitChromatographyBottled waterChemistryExtraction (chemistry)RT-qPCRBottled watermedicine.diseaseTiter030104 developmental biologylcsh:Biology (General)NorovirusViral hepatitisHepatitis E Virus (HEV)

description

Hepatitis E virus (HEV) is one of the causative agents of water-borne human viral hepatitis and considered in Europe an emerging zoonotic pathogen. Analysis of bottled water through a standard method validated for HEV can contribute towards the risk management of this hazard. Putting some recent reports by the European Food Safety Authority in place, this study aimed to assess the performance of the concentration and extraction procedures described in ISO 15216-1:2017 for norovirus and hepatitis A virus on HEV detection. Following the ISO recommendation, the bottled water samples were spiked using serially diluted HEV fecal suspensions together with mengovirus as process control and concentrated by filtration via positively charged nylon membranes. In order to extract viral RNA from the resulting concentrates, two different methods were compared in this study: The one recommended in the ISO norm, NucliSens&reg

yearjournalcountryeditionlanguage
2020-05-13
10.3390/microorganisms8050730http://hdl.handle.net/10261/212541