6533b85dfe1ef96bd12be8d7
RESEARCH PRODUCT
Elongation and desaturation of arachidonic and eicosapentaenoic acids in rat liver. Effect of clofibrate feeding
Jean-pierre PoissonJean Paul BlondLionel UlmannErling N. ChristiansenVirginie MimouniJean Bézardsubject
Male030309 nutrition & dieteticsBiophysicsMitochondria Liver[SDV.CAN]Life Sciences [q-bio]/CancerBiologyBiochemistry03 medical and health scienceschemistry.chemical_compoundEndocrinologymedicineAnimalsClofibrate[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM]ComputingMilieux_MISCELLANEOUS030304 developmental biologychemistry.chemical_classification0303 health sciencesClofibrateArachidonic AcidFatty acidPeroxisomeEicosapentaenoic acidRatschemistryBiochemistryEicosapentaenoic AcidLiverMicrosomeMicrosomes LiverFatty acid elongationArachidonic acidElongation[SDV.AEN]Life Sciences [q-bio]/Food and Nutritionmedicine.drugdescription
The fatty acid elongation-desaturation ability of 5,8,11,14-eicosatetraenoic (20:4(n-6)) and 5,8,11,14,17-eicosapentaenoic (20:5(n-3)) acids was determined in both liver microsomal and light mitochondrial (rich in peroxisomes) fractions of untreated and clofibrate treated rats. The elongation and the subsequent desaturation steps were performed in the corresponding favorable media. 20:5(n-3) elongation was about 2-times more extensive than that of 20:4(n-6). Clofibrate feeding for 10 days resulted in a marked decrease in the elongation rate with the two substrates, while the delta 4 desaturation rate was increased. There were small differences in the elongation rate between the microsomal and light mitochondrial fractions, however, the relative delta 4 desaturation rate was higher in the light mitochondrial fraction than microsomes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-11-01 |