6533b85dfe1ef96bd12be9f9
RESEARCH PRODUCT
Alpha-1-antitrypsin-induced inhibition of complement-dependent phagocytosis.
Anna MódManfred P. DierichJános GergelyGyorgy FüstSusan R. Hollánsubject
LeukemiaPhagocytosisImmunologyAlpha (ethology)Blood DonorsHematologyComplement receptorComplement C3Saccharomyces cerevisiaeCarbohydrateBiologyOpsonin ProteinsYeastPeripheral bloodMonocytesImmune systemBiochemistryPhagocytosisRosette formationalpha 1-AntitrypsinImmunology and AllergyHumansdescription
Abstract In a previous investigation, inhibition of complement-dependent rosette formation by alpha1-antitrypsin (α1-AT) was observed, and it was demonstrated that α1-AT interacts through its carbohydrate portion with C3 and its fragments. In the present study, the effect of α1-AT on the complement-receptor-mediated phagocytosis by human peripheral blood monocytes was examined. Purified α1-AT inhibited in a dose-dependent manner phagocytosis of C3-carrying yeast particles. Inhibition was selective, concerned only C3-receptor-mediated phagocytosis, neither Fc-receptor-mediated phagocytosis nor uptake of untreated yeast particles was blocked by α1-AT. It was demonstrated that α1-AT exerted its inhibitory effect through binding to C3-carrying particles. The activity of α1-AT towards C3 and fragments of C3 was not mediated by its antiprotease effect, but by its carbohydrate moiety. This finding suggests that α1-AT may have an impact on various immune functions involving complement receptors.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1981-04-01 | Immunobiology |