6533b85dfe1ef96bd12bf0cc
RESEARCH PRODUCT
pEGFP transfection into murine skeletal muscle by electrosonoporation
Uldis RubinsRoberts KadikisSaulius ŠAtkauskasMindaugas TamošiūnasDainis JakovelsRamona Petrovskasubject
Tibialis Cranialisanimal structuresChemistryvirusesElectroporationfungiSkeletal muscleTransfectionIn vitroGreen fluorescent proteinCell biologymedicine.anatomical_structureIn vivoembryonic structuresmedicineSonoporationdescription
In this study, we aimed to determine whether the combination of electroporation (EP) and ultrasound (US) waves (sonoporation) can affect the plasmid DNA transfection to mice tibialis cranialis muscle. Multispectral imaging technique combined with fluorescence spectroscopy point measurements has been used for the transcutaneous detection of enhanced green fluorescent protein (EGFP) fluorescence, providing information on location and duration of EGFP expression. We found that electrosonoporation, commonly enhancing pDNA transfection in vitro , had no positive effect on EGFP transfection efficiency increase in vivo with respect to electroporation alone. We presume that this may be associated with decreased viability of transfected fibers.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-12-07 | Biophotonics—Riga 2017 |