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RESEARCH PRODUCT
The kinetic behavior of insulin fibrillation is determined by heterogeneous nucleation pathways
Christian RischelChristian RischelFabio Librizzisubject
AmyloidProtein DenaturationTime FactorsAmyloidmedicine.medical_treatmentKineticsNucleationmacromolecular substancesProtein aggregationFibrilBiochemistryExponential growthmedicineAnimalsInsulinMolecular BiologyFibrillationChemistryInsulinTemperatureHydrogen-Ion ConcentrationKineticsBiochemistryFor the RecordBiophysicsCattlemedicine.symptomdescription
When subjected to acidic conditions and high temperature, insulin is known to produce fibrils that display the common properties of disease amyloids. Thus, clarifying the mechanisms of insulin fibrillation can help the general understanding of amyloidal aggregation. Insulin fibrillation exhibits a very sharp time dependence, with a pronounced lag phase and subsequent explosive growth of amyloidal aggregates. Here we show that the initial stages of this process can be well described by exponential growth of the fibrillated proteins. This indicates that the process is mainly controlled by a secondary nucleation pathway.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2005-12-01 | Protein Science |