6533b85efe1ef96bd12bf355
RESEARCH PRODUCT
Occurrence and genotypes of Giardia isolated from lambs in Spain
Rafael BorrásMaría Teresa Gómez-muñozMaría Magdalena Garijo-toledoMaría Auxiliadora Dea-ayuelaCristina NavarroMaría Teresa Pérez-graciaMaría Victoria Domínguez-márquezSalceda Fernández-barredosubject
GiardiasisVeterinary medicineGenotypeanimal diseasesMolecular Sequence DataHigh variabilityProtozoan ProteinsFluorescent Antibody TechniqueSheep DiseasesBiologyPolymerase Chain ReactionGenetic analysislaw.inventionMicrobiologyFeceslawZoonosesGenotypePrevalenceAnimalsPolymerase chain reactionSheepGiardiaGiardiaSequence Analysis DNAbiology.organism_classificationCytoskeletal ProteinsInfectious DiseasesSpainGiardia duodenalisParasitologyFlockdescription
Three hundred and eighty six faecal specimens were randomly collected from 1- to 3-month-old lambs from 16 farms in Spain to investigate the presence of different genotypes of Giardia duodenalis. Individual specimens were examined by IFA (Immunofluorescence assay) and beta-giardin PCR polymerase chain reaction. Cysts of G. duodenalis were shed by lambs in every flock analyzed, showing a prevalence by farms of 100%. The average prevalence of G. duodenalis for the 386 specimens was 42%, ranging from 8.3 to 80% depending on the farm. beta-giardin PCR positive samples were sequenced to determine the genotypes present at each farm and seven new subtypes of beta-giardin Assemblage E are reported in this study. In each farm, one to six different beta-giardin subtypes were found, showing the high variability of the target. Also, one flock had the zoonotic Assemblage A. This is the first report of Giardia subgenotype A-1 in sheep in Spain.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-01-04 | Parasitology International |