6533b85efe1ef96bd12bf492

RESEARCH PRODUCT

In vitro response of two Sicilian genotypes of Morus (L.) through axillary bud culture.

Maria GermanaPatricolo GermanaChiancone Benedetta

subject

Axillary buds in vitro culture micropropagationfood and beveragesBiologybiology.organism_classificationIn vitroSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeHorticultureMicropropagationAxillary budGenotypeShootBotanyGeneticsSubculture (biology)General Agricultural and Biological SciencesMorus nigraSprouting

description

A protocol for the in vitro establishment of two Sicilian genotypes of mulberry has been set up. Multiple shoots were initiated and plantlets were produced from buds collected from mature trees of Morus alba L. and Morus nigra L., grown in the field. The influence of two explanting times (September and December) of buds on the in vitro response has been observed. The percentage of sprouting after four weeks of culture was greatly higher with material explanted from the field in September compared with the one collected in December. Morus nigra L. produced more vigorous shoots than Morus alba L.. After the second subculture the number of shoots produced decreased, in both genotypes. No differ- ences were observed for both genotypes regarding the multiplication rate.

10.1080/00087114.2007.10589571http://hdl.handle.net/10447/9769