6533b85efe1ef96bd12bfd02
RESEARCH PRODUCT
Quality control of pharmaceuticals containing clenbuterol by thermal lens spectrometry.
J. J. Baeza BaezaY.martin BioscaGuillermo Ramis-ramossubject
Detection limitQuality ControlChromatographymedicine.diagnostic_testClinical BiochemistryPharmaceutical ScienceRepeatabilityAdrenergic beta-AgonistsMass spectrometryAnalytical ChemistryAbsorbancechemistry.chemical_compoundchemistryClenbuterolSpectrophotometryDrug DiscoverymedicineClenbuterolSpectrophotometry UltravioletDerivatizationQuantitative analysis (chemistry)Spectroscopymedicine.drugTabletsdescription
An ultrasensitive absorptiometric procedure for the determination of clenbuterol in pharmaceutical preparations was developed. Clenbuterol was diazotized with nitrite and coupled with 1-(naphthyl)ethylenediamine, and the absorbance of the azo dye formed was measured by both spectrophotometry and ultrasensitive thermal lens spectrometry (TLS). The TLS limit of detection was 1.5 ppb, 14-fold lower than with a Hewlett-Packard diode array spectrophotometer. Thus, the TLS procedure can be advantageously applied to quality control of clenbuterol at the individual dose level and in small samples. Repeatability as relative standard deviation was 1.5% (50 ppb, n = 6).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-06-01 | Journal of pharmaceutical and biomedical analysis |