6533b85efe1ef96bd12bfefb

RESEARCH PRODUCT

DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection

Marianne Smedegaard HedeGiovanni MarlettaPatrizia CancemiAlessio OttavianiYi-ping HoVittorio FerraraBirgitta R. KnudsenClaudia PelleritoAlessandro DesideriSalvatore FeoGiuseppe ArrabitoF CavaleriBruno Pignataro

subject

Materials scienceFlexible deviceNanotechnologymacromolecular substances02 engineering and technologySubstrate (printing)01 natural sciencesIndustrial and Manufacturing Engineeringchemistry.chemical_compoundA-DNALithographyTopoisomerasebiologyOligonucleotideTopoisomerase010401 analytical chemistrytechnology industry and agriculture021001 nanoscience & nanotechnology0104 chemical sciencesSettore BIO/18 - GeneticachemistryMolecular printingbiology.protein0210 nano-technologyBiosensorDNABiosensor

description

Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.

yearjournalcountryeditionlanguage
2019-01-18
10.1007/978-3-030-04324-7_39http://hdl.handle.net/10447/346288