6533b85efe1ef96bd12c05ae

RESEARCH PRODUCT

Novel porous carbon packings in reversed-phase high-performance liquid chromatography

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Abstract Porous carbon packings of particle size 5–20 μm were prepared by calcination of purified active carbons and cokes having narrow size ranges. Highly efficient columns packed with 10-μm particles were obtained by means of the high-viscosity slurry technique, and exhibited about 3000 theoretical plates at 100 mm length and optimal eluent flow-rate. The loading of columns, θ0.1, was determined to be 25–50 μg of solute per gram of packing. Eluotropic series of solvents were found to be similar to those on pyrocarbon-modified carbons and silicas. The log k′ values for homologous compounds increased linearly with the hydrocarbonaceous surface area at constant eluent composition. Studies with polar solutes indicated that the volume polarizability rather than dipole moment was the decisive molecular property of the solute with respect to retention. The successful application of carbon packings in aqueous organic eluents having a pH > 8.5 was demonstrated.