6533b85efe1ef96bd12c064e

RESEARCH PRODUCT

Synthetic Aperture Lensless Digital Holographic Microscopy (SALDHM) for Superresolved Biological Imaging

Luis GraneroJavier GarciaVicente MicóZeev Zalevsky

subject

DiffractionSynthetic aperture radarbusiness.industryHolographylaw.inventionOpticsExternal referencelawMicroscopic imagingDigital holographic microscopyBiological imagingbusinessHolographic recordingMathematics

description

Lensless digital holographic microscopy (LDHM) relates with the capability to achieve microscopic imaging working without lenses in the regime of holography. LDHM uses an extremely simplified setup and provides micron-range lateral and depth resolutions in three-dimensional (3D) imaging. Typically, LDHM uses a pinhole to provide spherical divergent illumination over the sample. Then, two different basic schemes are usually adopted for providing holographic recording. The first one assumes the interference between two in-line waves since diffraction by the sample can be considered as a perturbation of the reference wave. The second scheme uses an external reference beam in an off-line configuration extending the applicability of LDHM for any type of samples but increasing the complexity of the experimental layout. Nevertheless and in both cases, the sample phase distribution is recovered and becomes available for numerical refocusing and processing of the 3D sample.

https://doi.org/10.1016/b978-0-12-415871-9.00009-0