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RESEARCH PRODUCT
Induction of ambuic acid derivatives by the endophytic fungus Pestalotiopsis lespedezae through an OSMAC approach
Werner E. G. MüllerAttila MándiXiaoqin YuXiaoqin YuZhen LiuMarian FrankYing GaoTibor KurtánZhiyong GuoKun ZouRainer KalscheuerPeter ProkschPeter Prokschsubject
Natural productbiology010405 organic chemistryStereochemistryOrganic ChemistryFungus010402 general chemistrybiology.organism_classification01 natural sciencesBiochemistry0104 chemical sciencesAcinetobacter baumanniichemistry.chemical_compoundchemistryDrug DiscoveryFermentationCytotoxicityPestalotiopsisAntibacterial activityTwo-dimensional nuclear magnetic resonance spectroscopydescription
Abstract Ten new ambuic acid derivatives, pestallic acids H–Q including one new iodinated natural product along with two known compounds, ambuic acid and ambuic acid 18-acetate, were obtained through fermentation of the endophytic fungus Pestalotiopsis lespedezae on solid rice medium with 3.5% NaI. Pestallic acids H–Q were undetectable in cultures of the fungus grown on solid rice medium lacking NaI or in those where NaI had been replaced by NaCl or NaBr. The structures of the new metabolites were established on basis of 1D/2D NMR and HRESIMS data. Their absolute configurations were determined by Mosher’s method and TDDFT-ECD calculations. The compounds failed to show antibacterial activity against S. aureus (ATCC29213), drug-resistant Acinetobacter baumannii (BAA1605) or Mycobacterium tuberculosis, as well as cytotoxicity against the mouse lymphoma cell line L5178Y.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-01-01 | Tetrahedron |