6533b85ffe1ef96bd12c10f5
RESEARCH PRODUCT
Effects of dexamethasone on human synovial fibroblast-like cells, from osteoarthritic joints, in culture.
F. PiccioneMaria Valeria TorregrossaRenza VentoMichela GiulianoG. Grecomorosubject
endocrine systemmedicine.medical_specialtyTime FactorsCell divisionHydrocortisoneSomatic cellCell SurvivalCell CountBiologyGeneral Biochemistry Genetics and Molecular BiologyDexamethasoneCell Linechemistry.chemical_compoundInternal medicineOsteoarthritisSynovial Fluidpolycyclic compoundsmedicineSynovial fluidHumansGeneral Pharmacology Toxicology and PharmaceuticsFibroblastDexamethasoneCell growthGeneral MedicineDNAFibroblastsMolecular biologycultureEndocrinologymedicine.anatomical_structurechemistryCell cultureRNAThymidinehormones hormone substitutes and hormone antagonistsCell Divisionmedicine.drugdescription
The effect of Dexamethasone (DEX) on cell division and macromolecular synthesis was investigated in a line (McCoy cells, A 9) of synovial fibroblast-like cells derived from human osteoarthritic joints. DEX markedly reduced the proliferation of McCoy cells in a time and dose-dependent manner. The maximal inhibition (45%) was found at 500 nM DEX 24 h after incubation and was accompanied by the appearance of giant macrophage-like cells. After DEX treatment cells showed increased content of DNA, proteins and RNA together with the reduction of [3H]-thymidine incorporation into the TCA-precipitable fraction.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1990-01-01 | Life sciences |