6533b85ffe1ef96bd12c11ed

RESEARCH PRODUCT

FISH mapping of 18S-28S and 5S ribosomal DNA, (GATA)n and (TTAGGG)n telomeric repeats in the periwinkle Melarhaphe neritoides (Prosobranchia, Gastropoda, Caenogastropoda)

R BertoniAngelo LibertiniLuca CastriotaRoberto VitturiMariastella Colomba

subject

GeneticsCaenogastropodabiologyProsobranchiaRNA Ribosomal 5SChromosome MappingZoologyTelomerebiology.organism_classificationDNA RibosomalMolluscaMelarhaphe neritoidesRNA Ribosomal 28SGastropodaRNA Ribosomal 18SGeneticsAnimalsFish <Actinopterygii>Ribosomal DNAIn Situ Hybridization FluorescenceGenetics (clinical)Repetitive Sequences Nucleic Acid

description

Spermatocyte chromosomes of Melarhaphe neritoides (Mollusca, Prosobranchia, Caenogastropoda) were studied using fluorescent in situ hybridization (FISH) with four repetitive DNA probes (18S rDNA, 5S rDNA, (TTAGGG)n and (GATA)n). Single-colour FISH consistently mapped one chromosome pair per spread using either 18S or 5S rDNA as probes. The telomeric sequence (TTAGGG)n hybridized with termini of all chromosomes whereas the (GATA)n probe did not label any areas. Simultaneous 18S-5S rDNA and 18S-(TTAGGG)n FISH demonstrated that repeated units of the three multicopy families are closely associated on the same chromosome pair.

yearjournalcountryeditionlanguage
2001-08-14Heredity
https://doi.org/10.1038/sj.hdy.6800070