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RESEARCH PRODUCT
Primary Mucosal Epithelial Cell Cultivation: A Reliable and Accelerated Isolation
Sandra Nezi-cahnR SchwabStefan WalentaHeide-katharina BauerRonald E. UngerDaniela FleschAnnette HasenburgMartin HellerWalburgis Brennersubject
0303 health sciencesPrimary (chemistry)Tissue EngineeringIsolation (health care)0206 medical engineeringMouth MucosaBiomedical EngineeringMedicine (miscellaneous)Epithelial CellsBioengineeringCell Separation02 engineering and technologyBiology020601 biomedical engineeringEpitheliumCell biology03 medical and health sciencesmedicine.anatomical_structureCell cultureSoft tissue engineeringmedicineHumansCells Cultured030304 developmental biologydescription
We illustrate a reliable and accelerated isolation routine for mucosal epithelial cells, which thereupon can be used for soft tissue engineering. This is highly important in the field of soft tissue engineering because mucosal equivalents are frequently usable in several surgical fields like gynecology, urology, otorhinolaryngology, ophthalmology, maxillofacial surgery, and many others. In this context the isolation of mucosal epithelial cells suitable for tissue engineering is mandatory. The reliable cultivation of mucosal or skin epithelial cells is challenging and there is currently no reproducible method. We demonstrate a solution for this problem by developing an accelerated and nevertheless reliable method.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-02-01 | Tissue Engineering Part C: Methods |