6533b85ffe1ef96bd12c1d96

RESEARCH PRODUCT

Development of Antioxidant Protein Extracts from Gilthead Sea Bream (Sparus aurata) Side Streams Assisted by Pressurized Liquid Extraction (PLE)

Beatriz De La FuenteFrancisco J. BarbaNoelia PallarésHouda Berrada

subject

Fish ProteinsSpectrometry Mass Electrospray IonizationAntioxidantOxygen radical absorbance capacityFood HandlingElectrospray ionizationmedicine.medical_treatmentLiquid-Liquid ExtractionTrolox equivalent antioxidant capacityPharmaceutical Scienceantioxidant capacity01 natural sciencesAntioxidantsArticle0404 agricultural biotechnologymycotoxinsDrug Discoverypressurized liquid extractionmedicinePressuregilthead sea breamAnimalsSea bassheavy metalsPharmacology Toxicology and Pharmaceutics (miscellaneous)Inductively coupled plasma mass spectrometrylcsh:QH301-705.5Chromatography High Pressure LiquidWaste ProductsChromatographyOxygen Radical Absorbance CapacitybiologyChemistryside streams010401 analytical chemistryExtraction (chemistry)04 agricultural and veterinary sciencesbiology.organism_classification040401 food scienceSea Bream0104 chemical sciencesMolecular Weightlcsh:Biology (General)MetalsDicentrarchusElectrophoresis Polyacrylamide GelproteinSDS-PAGE

description

The pressurized liquid extraction (PLE) technique was used, for the first time, to obtain protein extracts with antioxidant activity from side streams (muscle, heads, viscera, skin, and tailfins) of gilthead sea bream (Sparus aurata) in order to give added value to these underutilized matrices. Extraction conditions previously optimized for sea bass (Dicentrarchus labrax) side streams were applied. Protein recovery percentages were 22% (muscle), 33% (heads), 78% (viscera), 24% (skin), and 26% (tailfins), which represented an increase of 1.2–4.5-fold compared to control samples (extraction by stirring). The SDS-PAGE profiles revealed that PLE-assisted extraction influenced protein molecular weight distribution of the obtained extracts. PLE conditions also allowed increasing the antioxidant capacity measured by both Trolox equivalent antioxidant capacity (TEAC

yearjournalcountryeditionlanguage
2021-04-01Marine Drugs
10.3390/md19040199http://europepmc.org/articles/PMC8066512