6533b85ffe1ef96bd12c2545

RESEARCH PRODUCT

Reversible effect of magnetic fields on human lymphocyte activation patterns: different sensitivity of naive and memory lymphocyte subsets.

Marco Pio La MannaCarmela La MendolaNadia CaccamoAlfredo SalernoAntonio Lo CastoSergio Salerno

subject

AdultCD4-Positive T-LymphocytesMaleTime Factorsmedicine.medical_treatmentBiophysicsBiologyLymphocyte ActivationInterferon-gammaMagneticsCytosolstatic magnetic fields CD4(+) T cells.T-Lymphocyte SubsetsmedicineHumansRadiology Nuclear Medicine and imagingCells CulturedCell ProliferationCalcium metabolismHuman lymphocyteRadiationCell growthMagnetic fieldCell biologyCytokineCell cultureImmunologyLeukocyte Common AntigensCalciumFemaleShort exposureImmunologic MemoryLymphocyte subsets

description

The aim of this study was to investigate the influence of 50 Hz magnetic or static magnetic fields of 0.5 mT on subsets of human CD4(+) T cells in terms of cytokine release/content, cell proliferation and intracellular free calcium concentration. CD4(+) T cells can be divided into different subsets on the basis of surface marker expression, such as CD45, and T cells can be divided into naive (CD45RA(+)) and memory (CD45RA(-)) cells. In this study, the effects of magnetic fields after 24 and 48 h of cell culture were analyzed. We found that the CD4(+)CD45RA(-) T subset were more sensitive after 2 h of exposure. Decreases in the release/content of IFN-gamma, in cell proliferation and in intracellular free calcium concentrations were observed in exposed CD4(+)CD45RA(-) T cells compared to CD4(+)CD45RA(+) T cells. The results suggest that exposure to the magnetic fields induces a delay in the response to stimulants and that modifications are rapidly reversible, at least after a short exposure.

yearjournalcountryeditionlanguage
2009-10-01
10.1667/rr1761.1http://hdl.handle.net/10447/44939