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RESEARCH PRODUCT
Detection and Characterization of Wolbachia Infections in Natural Populations of Aphids: Is the Hidden Diversity Fully Unraveled?
Paulo A. V. BorgesVangelis DoudoumisVangelis DoudoumisManhaz KhademSilvia RamosMarios ScarvelakisAristeidis PapapanagiotouMarta MoreiraAntónio F. AguiarGeorge TsiamisGeorge TsiamisAntonis A. AugustinosAntonis A. AugustinosKostas BourtzisKostas BourtzisDiego Santos-garciaAmparo LatorreEva DionyssopoulouEva Dionyssopoulousubject
0106 biological sciences[SDV]Life Sciences [q-bio]FaunaPopulation DynamicsBiological pest controllcsh:MedicineWolbachia InfectionsPolymerase Chain Reaction01 natural sciencesRNA Ribosomal 16SGenotypelcsh:SciencePhylogenyreproductive and urinary physiologyGenetics0303 health sciencesMultidisciplinaryfood and beveragesAgricultureWolbachiaWolbachiaResearch ArticleGenotypeMolecular Sequence DataAgro-Population EcologyZoologyBiologyDNA RibosomalMicrobiology010603 evolutionary biologyMicrobial Ecology03 medical and health sciencesPhylogeneticsparasitic diseasesGenetic variationAnimalsBiologyAlleles030304 developmental biologyEvolutionary Biologylcsh:RBacterial TaxonomyGenetic VariationBacteriologybiochemical phenomena metabolism and nutritionRibosomal RNAbiology.organism_classificationOrganismal EvolutionAphidsMicrobial EvolutionbacteriaMultilocus sequence typinglcsh:QGram-Negative Bacterial InfectionsZoologyEntomologyAgroecologyMultilocus Sequence Typingdescription
Copyright © 2011 Augustinos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Aphids are a serious threat to agriculture, despite being a rather small group of insects. The about 4,000 species worldwide engage in highly interesting and complex relationships with their microbial fauna. One of the key symbionts in arthropods is Wolbachia, an a-Proteobacterium implicated in many important biological processes and believed to be a potential tool for biological control. Aphids were thought not to harbour Wolbachia; however, current data suggest that its presence in aphids has been missed, probably due to the low titre of the infection and/or to the high divergence of the Wolbachia strains of aphids. The goal of the present study is to map the Wolbachia infection status of natural aphids populations, along with the characterization of the detected Wolbachia strains. Out of 425 samples from Spain, Portugal, Greece, Israel and Iran, 37 were found to be infected. Our results, based mainly on 16S rRNA gene sequencing, indicate the presence of two new Wolbachia supergroups prevailing in aphids, along with some strains belonging either to supergroup B or to supergroup A.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-12-13 | PLoS ONE |