6533b85ffe1ef96bd12c262d
RESEARCH PRODUCT
In Vitro–In Vivo Fluctuation Spectroscopies
Massimo PanzicaMichele CacciaMaddalena ColliniIlaria RivoltaB. LettieroLaura SironiGiuseppe ChiricoLaura D'alfonsoGiuseppe Miserocchisubject
Materials scienceField (physics)Anomalous diffusioncorrelation spectroscopyFluorescence correlation spectroscopySettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)Fluorescence Correlation Spectroscopy FCS Spectroscopies vitro vivo cell single molecule fluctuation FFS nanoparticle.Dynamic light scatteringChemical physicsMicroscopymicroscopyfluorescenceDiffusion (business)SpectroscopyTwo-dimensional nuclear magnetic resonance spectroscopydescription
Fluorescence correlation spectroscopy (FCS) was first developed for biophysical studies in analogy with photon scattering correlation spectroscopy. Although it is mainly devoted to the study of freely diffusing particles, FCS is actually able to discern between different kinds of motions, such as diffusion, anomalous diffusion, or drift motions. The frontier application of FCS nowadays is in medical studies both within cells and on the cell membranes, and in the investigation of single molecules in solid matrices. In this field, FCS originated also image correlation spectroscopy methods. The whole field can be unified under the name of fluorescence fluctuation spectroscopy (FFS). We present here a short review of the theoretical bases of FFS under a unified vision and discuss some applications to the study of dynamics of nanoparticles in cells and to the investigation of the photodynamics of immobilized dyes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-11-01 |