6533b85ffe1ef96bd12c2678
RESEARCH PRODUCT
Low testosterone levels are related to oxidative stress, mitochondrial dysfunction and altered subclinical atherosclerotic markers in type 2 diabetic male patients
Milagros RochaNoelia Diaz-moralesCelia BañulsSusana Rovira-llopisVictor M. VictorAranzazu M. De MarañónAntonio Hernández-mijaresSandra GarzonAna Joversubject
AdultMaleRiskMitochondrial ROSmedicine.medical_specialtyApolipoprotein BVascular Cell Adhesion Molecule-1030209 endocrinology & metabolismInflammationType 2 diabetes030204 cardiovascular system & hematologyMitochondrionmedicine.disease_causeBiochemistry03 medical and health sciences0302 clinical medicinePhysiology (medical)Internal medicineLeukocytesmedicineHumansTestosteroneMembrane Potential Mitochondrialchemistry.chemical_classificationReactive oxygen speciesbiologyTestosterone (patch)Middle AgedAtherosclerosismedicine.diseaseMitochondriaOxidative StressEndocrinologyDiabetes Mellitus Type 2chemistrybiology.proteinCytokinesInflammation Mediatorsmedicine.symptomReactive Oxygen SpeciesBiomarkersOxidative stressdescription
Abstract Introduction Low testosterone levels in men are associated with type 2 diabetes and cardiovascular risk. However, the role of testosterone in mitochondrial function and leukocyte-endothelium interactions is unknown. Our aim was to evaluate the relationship between testosterone levels, metabolic parameters, oxidative stress, mitochondrial function, inflammation and leukocyte-endothelium interactions in type 2 diabetic patients. Materials and methods The study was performed in 280 male type 2 diabetic patients and 50 control subjects. Anthropometric and metabolic parameters, testosterone levels, reactive oxygen species (ROS) production, mitochondrial membrane potential, TNFα, adhesion molecules and leukocyte-endothelium cell interactions were evaluated. Results Testosterone levels were lower in diabetic patients. Total and mitochondrial ROS were increased and mitochondrial membrane potential, SOD and GSR expression levels were reduced in diabetic patients. TNFα, ICAM-1 and VCAM-1 levels, leukocyte rolling flux and adhesion were all enhanced in diabetic patients, while rolling velocity was reduced. Testosterone levels correlated negatively with glucose, HOMA-IR, HbA1c, triglycerides, nonHDL-c, ApoB, hs-CRP and AIP, and positively with HDL-c and ApoA1. The multivariable regression model showed that HDL-c, HOMA-IR and age were independently associated with testosterone. Furthermore, testosterone levels correlated positively with membrane potential and rolling velocity and negatively with ROS production, VCAM-1, rolling flux and adhesion. Conclusions Our data highlight that low testosterone levels in diabetic men are related to impaired metabolic profile and mitochondrial function and enhanced inflammation and leukocyte-endothelium cell interaction, which leaves said patients at risk of cardiovascular events.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-01-03 | Free Radical Biology and Medicine |