6533b85ffe1ef96bd12c2731

RESEARCH PRODUCT

Specific Detection and Localization of Microsporidian Parasites in Invertebrate Hosts by Using In Situ Hybridization

Leellen F. SolterHenk R. BraigAurore DubuffetAurore DubuffetAlison M. DunnM. Alejandra PerottiM. Alejandra PerottiJudith E. Smith

subject

In situ[ SDV.MP.PAR ] Life Sciences [q-bio]/Microbiology and Parasitology/ParasitologyVairimorphaZoologyIn situ hybridizationMicrobiology[SDV.MP.PRO]Life Sciences [q-bio]/Microbiology and Parasitology/Protistology[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunityApplied Microbiology and BiotechnologyMicrobiology03 medical and health sciencesparasitic diseases[ SDV.EE.IEO ] Life Sciences [q-bio]/Ecology environment/SymbiosisInvertebrate MicrobiologymedicineAnimalsParasite hostingAmphipoda[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/ParasitologyIn Situ Hybridization FluorescenceComputingMilieux_MISCELLANEOUS030304 developmental biologyInvertebrate[ SDE.BE ] Environmental Sciences/Biodiversity and Ecology0303 health sciencesEcologybiologymedicine.diagnostic_test030306 microbiology[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]fungibiology.organism_classification3. Good healthNosemaMicrosporidiaMicrosporidiaOligonucleotide Probes[SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/SymbiosisFood ScienceBiotechnologyFluorescence in situ hybridization

description

ABSTRACT We designed fluorescence in situ hybridization probes for two distinct microsporidian clades and demonstrated their application in detecting, respectively, Nosema / Vairimorpha and Dictyoceola species. We used them to study the vertical transmission of two microsporidia infecting the amphipod Gammarus duebeni .

yearjournalcountryeditionlanguage
2013-01-01Applied and Environmental Microbiology
https://doi.org/10.1128/aem.02699-12