Recombinant cDNA encapsulation in small liposomes with hepatocyte access ability.

6533b85ffe1ef96bd12c282e

RESEARCH PRODUCT

Recombinant cDNA encapsulation in small liposomes with hepatocyte access ability.

Salvador F. Aliño M. Lejarreta M. Bobadilla Manuel Garcia-sanz Enrique Hilario Fernando Unda

subject

Drug Compounding DNA Recombinant Pharmaceutical Science Bioengineering Biology In Vitro Techniques law.invention chemistry.chemical_compound Mice Colloid and Surface Chemistry law Complementary DNA medicine Animals Humans Physical and Theoretical Chemistry Fluorescein Particle Size Liposome Drug Carriers Chromatography Paraffin Embedding Staining and Labeling Organic Chemistry Fluoresceins Mice Inbred C57BL Microscopy Electron medicine.anatomical_structure Biochemistry chemistry Liver Hepatocyte alpha 1-Antitrypsin Liposomes Recombinant DNA Extrusion Particle size Drug carrier Filtration Plasmids

description

Liposomal encapsulation efficiency of a recombinant cDNA was studied by several procedures. We observed that supernatant fraction of ultracentrifuged liposomes prepared by extrusion through polycarbonate filters of 400 nm pore size yielded a very homogeneous suspension of small (50 nm diameter) unilamellar liposomes with highest DNA/lipid ratio and great ability to access to hepatocytes.

year	journal	country	edition	language
1993-04-01	Journal of microencapsulation

10.3109/02652049309104382 https://pubmed.ncbi.nlm.nih.gov/7687286

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