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RESEARCH PRODUCT

Detection of oncogenic human papillomavirus genotypes on spermatozoa from male partners of infertile couples

Giuseppa CapraRenato VeneziaGiovanni RuvoloRosaria Schillaci 6 Concetta ScazzoneC BellaviaAntonio Perino

subject

AdultMaleendocrine systemmedicine.medical_specialtyGenotypeReproductive medicineSemenFertilization in VitroBiologyPolymerase Chain ReactionHuman Papillomavirus DNA TestsMale infertilityAndrologySemen qualityPredictive Value of TestsRisk FactorsGenotypemedicineHumansPapillomaviridaeIn Situ HybridizationInfertility MaleAcademic Medical CentersSperm Counturogenital systemPapillomavirus InfectionsHPV infectionvirus diseasesObstetrics and Gynecologymedicine.diseaseSpermatozoaSpermSettore MED/40 - Ginecologia E Ostetriciafemale genital diseases and pregnancy complicationsFertilityReproductive MedicineCase-Control StudiesDNA ViralSperm MotilitySperm HeadHPV infection semen parameters IVF ICSINested polymerase chain reaction

description

Objective To evaluate the prevalence of human papillomavirus (HPV) sperm infection and its correlation with sperm parameters in patients who attended a fertility clinic. Design Cross-sectional clinical study. Setting University-affiliated reproductive medicine clinic. Patient(s) A total of 308 male partners of couples undergoing in vitro fertilization techniques. Intervention(s) Specimens of semen were collected from all patients. Main Outcome Measure(s) Sperm parameters were evaluated according to the World Health Organization manual. The presence of HPV DNA was researched by the combined use of two HPV assays and a highly sensitive nested polymerase chain reaction assay followed by HPV genotyping. To examine whether HPV was associated with the sperm, in situ hybridization (ISH) analysis was performed. Result(s) Results of HPV investigation were compared with sperm parameters and ISH analysis. Twenty-four out of 308 semen samples (7.8%) were HPV DNA positive, but HPV infection did not seem to affect semen quality. Moreover, ISH revealed a clear HPV localization at the equatorial region of sperm head in infected samples. Conclusion(s) Oncogenic HPV genotypes were detected on spermatozoa from asymptomatic subjects, but a role of the infection in male infertility was not demonstrated.

yearjournalcountryeditionlanguage
2013-11-01
http://hdl.handle.net/10447/93304