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RESEARCH PRODUCT

Liquid chromatographic determination of Vitamin D3 in infant formulas and fortified milk

Sara PeralesRosaura FarréAmparo AlegríaReyes BarberáM.m. Delgado

subject

VitaminDetection limitChromatographyVitamina dBiochemistryAnalytical ChemistryVitamin d 3chemistry.chemical_compoundFortified milkchemistryInfant formulaVitamin D and neurologyEnvironmental ChemistryTrace analysisSpectroscopy

description

Abstract An isocratic reverse-phase liquid chromatographic (LC) method with electrochemical (EC) detection has been developed and validated for determining Vitamin D 3 in fortified milk and infant formulas. LC–EC determination provides linear responses in the range from 0.03 to 0.7 μg Vitamin D 3 /ml, with inter- and intra-day variations (R.S.D.%) of 4.1 and 4.4, respectively, and detection and quantification limits of 0.012 ng in assay (48 ng/100 g of sample) and 0.039 ng in assay (156 ng/100 g of sample), respectively. Application of the method to a set of infant formulas and fortified milk confirmed its usefulness.

yearjournalcountryeditionlanguage
2005-07-01Analytica Chimica Acta
https://doi.org/10.1016/j.aca.2005.04.019