6533b860fe1ef96bd12c3028

RESEARCH PRODUCT

Efficient Production of Isoflavonoids by Astragalus membranaceus Hairy Root Cultures and Evaluation of Antioxidant Activities of Extracts

Thomas EfferthYu-jie FuXiao PengWei MaShengnan TanJiao JiaoQing-yan Gai

subject

ChromatographyAntioxidantbiologymedicine.medical_treatmentCell Culture TechniquesGeneral ChemistryAstragalus propinquusbiology.organism_classificationIsoflavonesPlant RootsAntioxidantsAstragalusNutraceuticalDry weightLc ms msmedicineIc50 valuesFood scienceGeneral Agricultural and Biological SciencesDrugs Chinese Herbal

description

In this study, Astragalus membranaceus hairy root cultures (AMHRCs) were established as an attractive alternative source for the efficient production of isoflavonoids (IF). A. membranaceus hairy root line II was screened as the most efficient line and was confirmed by PCR amplification of rolB, rolC and aux1 genes. Culture parameters of AMHRCs were systematically optimized, and five main IF constituents were quali-quantitatively determined by LC-MS/MS. Under optimal conditions, the total IF accumulation of 34 day old AMHRCs was 234.77 μg/g dry weight (DW). This yield was significantly higher compared to that of 3 year old field grown roots (187.38 μg/g DW). Additionally, in vitro antioxidant assays demonstrated that AMHRC extracts exhibited antioxidant activities with lower IC50 values (1.40 and 1.73 mg/mL) as compared to those of field grown roots (1.96 and 2.17 mg/mL). Overall, AMHRCs may offer a promising and continuous product platform for naturally derived, high quality and valuable nutraceuticals.

yearjournalcountryeditionlanguage
2014-12-09Journal of Agricultural and Food Chemistry
https://doi.org/10.1021/jf503839m