6533b860fe1ef96bd12c3976
RESEARCH PRODUCT
Applications of Super Resolution Expansion Microscopy in Yeast
Liwen ChenLongfang YaoLi ZhangYiyan FeiLan MiJiong MaJiong MaJiong Masubject
Fluorescence-lifetime imaging microscopyMaterials scienceMaterials Science (miscellaneous)BiophysicsGeneral Physics and Astronomyyeastlaw.invention03 medical and health sciences0302 clinical medicineOptical microscopelawnuclear pore complexMicroscopySample preparationPhysical and Theoretical ChemistryseptinMathematical Physics030304 developmental biology0303 health sciencesResolution (electron density)expansion super-resolutionImaging studySuperresolutionYeastlcsh:QC1-999tubulinBiological system030217 neurology & neurosurgerylcsh:Physicsdescription
Super-resolution microscopy includes multiple techniques in optical microscopy that enable sub-diffraction resolution fluorescence imaging of cellular structures. Expansion microscopy (EXM) is a method of physical expansion to obtain super-resolution images of a biological sample on conventional microscopy. We present images of yeast organelles, applying the combination of super-resolution and ExM techniques. When preparing pre-expanded samples, conventional methods lead to breakage of dividing yeast cells and difficulties in studying division-related proteins. Here, we describe an improved sample preparation technique that avoids such damage. ExM in combination with Airyscan and structured illumination microscopy (SIM) collected sub-cellular structural images of nuclear pore complex, septin, and a-tubulin in yeast. Our method of expansion in yeast is well-suited for super-resolution imaging study of yeast.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-03-01 | Frontiers in Physics |