6533b860fe1ef96bd12c3a47
RESEARCH PRODUCT
Evidence for specific, high-affinity binding sites for a proteinaceous elicitor in tobacco plasma membrane
Jean-pierre BleinPierre RicciAlain PuginMarie-noëlle BinetDavid Wendehennesubject
0106 biological sciencesNicotiana tabacumBiophysics[SDV.BC]Life Sciences [q-bio]/Cellular Biology01 natural sciencesBiochemistryFungal Proteins03 medical and health sciencesStructural BiologyIn vivoTobaccoGeneticsBinding siteReceptor[SDV.BC] Life Sciences [q-bio]/Cellular BiologyMolecular BiologyComputingMilieux_MISCELLANEOUS030304 developmental biology0303 health sciencesBinding SitesbiologyNicotiana tabacumChemistryAlgal ProteinsCell MembraneElicitinBiological activityCell BiologyElicitorbiology.organism_classification3. Good healthElicitorKineticsPlants ToxicMembraneBiochemistryCryptogeinPlasma membraneReceptor010606 plant biology & botanydescription
Abstract Binding of cryptogein, a proteinaceous elicitor, was studied on tobacco plasma membrane. The binding of the [125I]cryptogein was saturable, reversible and specific with an apparent Kd of 2 nM. A single class of cryptogein binding sites was found with a sharp optimum pH for binding at about pH 7.0. The high-affinity correlates with cryptogein concentrations required for biological activity in vivo.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-10-30 |