COX-2 ekspresijas inducēšana LPS iekaisuma modelī neiroblastomas SH-SY5Y šūnu līnijā

6533b860fe1ef96bd12c3da1

RESEARCH PRODUCT

COX-2 ekspresijas inducēšana LPS iekaisuma modelī neiroblastomas SH-SY5Y šūnu līnijā

subject

LPS imūnfluorescence Farmācija SH-SY5Y šūnas COX-2 iekaisums

description

COX-2 ir COX izoenzīms, kas atbild par arahidonskābes metabolismu, sintezējot bioloģiski aktīvus iekaisuma mediatorus – prostanoīdus. Perifērijā COX-2 ir inducējama izoforma, bet smadzenēs – konstitutīva. Šī darba mērķis bija izpētīt LPS spēju inducēt COX-2 ekspresiju neiroblastomas SH-SY5Y šūnās. COX-2 ekspresijas noteikšanai tika izmantota imūnfluorescences metode. Rezultāti uzrādīja SH-SY5Y šūnu morfoloģijas izmaiņas dažādu LPS koncentrāciju (5 – 800 ng/ml) ietekmē. Pētījumā novēroja COX-2 ekspresiju kontroles šūnās normālos apstākļos, LPS koncentrācijā 5 ng/ml COX-2 ekspresijas indukciju, bet koncentrācijā 25 ng/ml un 50 ng/ml – supresiju. Statistiski ticamu rezultātu uzrādīja LPS koncentrācijā 50 ng/ml.

year	journal	country	edition	language
2015-01-01

https://dspace.lu.lv/dspace/handle/7/29182

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