6533b861fe1ef96bd12c4536
RESEARCH PRODUCT
IgG anti-endothelial cell antibodies (AECA) in type I diabetes mellitus; induction of adhesion molecule expression in cultured endothelial cells
G TrioloG TrioloA Accardo-palumboM C CarboneA FerranteD CasigliaE Giardinasubject
medicine.medical_specialtyAdolescentNeutrophilsImmunologyIntercellular Adhesion Molecule-1GranulocyteUmbilical veinInternal medicineE-selectinCell AdhesionmedicineHumansImmunology and AllergyChildCells CulturedAutoantibodiesType 1 diabetesbiologybusiness.industryCell adhesion moleculeIntercellular Adhesion Molecule-1medicine.diseaseEndothelial stem cellDiabetes Mellitus Type 1medicine.anatomical_structureEndocrinologyChild PreschoolImmunoglobulin GImmunologybiology.proteinOriginal ArticleEndothelium VascularAntibodyE-Selectinbusinessdescription
SUMMARYAECA were detected in 25 of 71 patients with type 1 diabetes mellitus and in two of 33 healthy subjects. Patients with diabetes of < 1 year duration and those with long-standing disease had the highest levels of these antibodies. Inhibition studies suggest that at least part of the AECA reactivity is due to cross-reactive anti-ssDNA antibodies. AECA-positive sera were able to increase intercellular adhesion molecule-1 (ICAM-1) and E-selectin on human umbilical vein endothelial cells (HUVEC). Increased binding of polymorphonuclear (PMN) cells was also found to accompany raised E-selectin expression. Soluble ICAM-1 and E-selectin were also found to be increased in the sera of AECA-positive patients. An effect of AECA on endothelial cell function is suggested in diabetes mellitus.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-03-01 | Clinical and Experimental Immunology |