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RESEARCH PRODUCT

AB0919 H-Ferritin and CD68+/H-ferritin+ Cells Are Increased in The Skin of Adult Onset Still's Disease Patients and Correlate with The Disease Activity

Roberto GiacomelliGiuliana GugginoPiero RuscittiOnorina BerardicurtiS Di BartolomeoFrancesco CarubbiFrancesco CicciaPaola CiprianiV. LiakouliG. TrioloP. Di Benedetto

subject

0301 basic medicineErythemabiologyCD68Septic shockbusiness.industryImmunologyArthritismedicine.disease_causemedicine.diseaseGeneral Biochemistry Genetics and Molecular BiologyAutoimmunityProinflammatory cytokineFerritin03 medical and health sciences030104 developmental biologyRheumatologyMacrophage activation syndromeImmunologymedicinebiology.proteinImmunology and Allergymedicine.symptombusiness

description

Background Adult onset Still9s disease (AOSD) is an inflammatory disease, characterized by high spiking fevers, arthritis, salmon-pink erythema and multivisceral involvement [1]. During AOSD, exceptionally high serum levels of ferritin may be observed and they might contribute to production of proinflammatory molecules [2]. Ferritin is composed by 24 subunits, heavy (H) subunits and light (L) subunits. The ferritin enriched in L subunits (L-ferritin) and the ferritin enriched in H subunits (H-ferritin) may be recognized in different tissues [3]. Objectives To investigate the skin tissue expression of both H-and L-ferritin and the number of macrophages expressing these molecules, in the inflammatory cutaneous infiltrate from 10 AOSD patients with persistent (more than 24–36 hours) cutaneous lesions. In addition, we performed statistical analysis to evaluate whether these results might correlate with the severity of the disease. Methods We obtained skin samples from 10 consecutive patients with active AOSD with persistent cutaneous lesions and 10 healthy controls (HC). An immunofluorescence analysis was performed evaluating the tissue expression of both H- and L-ferritin and the number of macrophages expressing these moleculkes. Serum levels of ferritin, ESR, CRP, Pouchot9s score were also recorded. Results We observed an increased H-ferritin expression in the skin samples of AOSD patients, when compared with HC (p Conclusions Our data show an imbalance between the production of L- and H-ferritin in the skin of AOSD patients, associated with a strong infiltrate of CD68+/H-ferritin+ cells in the same samples suggesting a possible pathogenic role. Furthermore, a correlation between the levels of H-ferritin in the skin of these patients and the disease activity was observed. References Gerfaud-Valentin M, et al. (2014) Adult-onset Still9s disease. Autoimmun Rev 13:708–22. Rosario C, et al (2013) The hyperferritinemic syndrome: macrophage activation syndrome, Still9s disease, septic shock and catastrophic antiphospholipid syndrome. BMC Med 11:185. Recalcati S, et al (2008) New functions for an iron storage protein: the role of ferritin in immunity and autoimmunity. J Autoimmun 30:84–9. Disclosure of Interest None declared

https://doi.org/10.1136/annrheumdis-2016-eular.2616