6533b861fe1ef96bd12c5802

RESEARCH PRODUCT

Thermal inactivation kinetics of Bacillus stearothermophilus spores using a linear temperature program.

Avelina FernándezAntonio MartínezCarmen RodrigoS. LeontidisL. MagranerP.s Fernández

subject

Spores BacterialHot TemperaturebiologyChemistryfungiKineticsColony Count MicrobialTemperatureBacillusThermodynamicsbiology.organism_classificationKinetic energyMicrobiologyEndosporeMicrobiologySporeDisinfectionGeobacillus stearothermophilusThermalZ-valueNonlinear regressionFood Science

description

A systematic study of the inactivation kinetics of Bacillus stearothermophilus spores was carried out in nonisothermic heating conditions using a linear temperature increase program and analyzing the experimental data by means of a one-step nonlinear regression. The D and z values estimated are close to those obtained in isothermic conditions and estimated by using a two-step model, first D values are calculated, and then in the second step a z value is deduced (D(121 degrees C) = 3.08 and 4.38 min, respectively, and z = 7 and 7.9 degrees C, respectively). No convergence problems were observed when using the one-step nonlinear regression proposed. The results indicated that the methodology applied in this study can be used to obtain kinetic data for bacterial spores, which could mean a significant reduction in the amount of experimental work employed to generate these data.

10.4315/0362-028x-62.8.958https://pubmed.ncbi.nlm.nih.gov/10456754