6533b861fe1ef96bd12c583f
RESEARCH PRODUCT
Enhanced expression of IL-8 in normal human keratinocytes and human keratinocyte cell line HaCaT in vitro after stimulation with contact sensitizers, tolerogens and irritants.
Joachim SalogaAlexander EnkMansour MohamadzadehJürgen KnopThomas HultschMarkus Miillersubject
KeratinocytesCatecholsStimulationDermatologyDermatitis ContactBiochemistryGene expressionmedicineImmune ToleranceHumansInterleukin 8Northern blotRNA MessengerMolecular BiologyCells CulturedCell Line TransformedChemistryInterleukin-8Sodium Dodecyl SulfateMolecular biologyIn vitroHaCaTmedicine.anatomical_structureGene Expression RegulationCell cultureImmunologyIrritantsDinitrofluorobenzeneKeratinocytedescription
Abstract To investigate the interleukin-8 production of keratinocytes after stimulation in vitro we have used various agents: (i) contact sensi-tizer (2,4-dinitrofiuorobenzene, 3-n-penladecylcatechol); (ii) tolerogen (5-methyl-3-n-pentadecylcatechol); (iii) irritant (sodium lauryl sulfate). Interleukin-8 gene expression was assessed by northern blot hybridization of the total cytoplasmic RNA extracted from subconfluent normal human keratinocyte cultures and the keratinocyte cell line HaCaT using a radiolabeled DNA probe specific for human interleukin-8. Intcrleukin-8 gene expression was markedly increased upon in vitro stimulation after 1-6 h with contact sensitizers, tolerogen and the irritant. In contrast, in-terlcukin-8 production was not detectable in unstimulatcd normal human keratinocytes or the HaCaT keratinocyte cell line. These results suggest that the induction and production of interleukin-8 is a response to nonspecific stimuli and may play a critical role in the early response to immuno-genic or inflammatory signals in man.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-12-01 | Experimental dermatology |