6533b861fe1ef96bd12c584f

RESEARCH PRODUCT

Gene toxicity studies on titanium dioxide and zinc oxide nanomaterials used for UV-protection in cosmetic formulations

Karin WienchMarkus SchulzBen Van RavenzwaayStefan SchulteFranz OeschLan Ma-hockRobert LandsiedelWendel WohllebenSamantha Champ

subject

MaleMaterials scienceBiomedical EngineeringBone Marrow CellsNanotechnologyCosmeticsGene mutationToxicologymedicine.disease_causeCell LineNanomaterialsMicechemistry.chemical_compoundSalmonellaIn vivoCricetinaeAdministration InhalationMacrophages AlveolarmedicineAnimalsRats WistarMicronuclei Chromosome-DefectiveTitaniumChromatographyMutagenicity TestsBody WeightIn vitroNanostructuresRatschemistryData Interpretation StatisticalMicronucleus testTitanium dioxideZinc OxideMicronucleusSunscreening AgentsGenotoxicity

description

Titanium dioxide and zinc oxide nanomaterials, used as UV protecting agents in sunscreens, were investigated for their potential genotoxicity in in vitro and in vivo test systems. Since standard OECD test methods are designed for soluble materials and genotoxicity testing for nanomaterials is still under revision, a battery of standard tests was used, covering different endpoints. Additionally, a procedure to disperse the nanomaterials in the test media and careful characterization of the dispersed test item was added to the testing methods. No genotoxicity was observed in vitro (Ames' Salmonella gene mutation test and V79 micronucleus chromosome mutation test) or in vivo (mouse bone marrow micronucleus test and Comet DNA damage assay in lung cells from rats exposed by inhalation). These results add to the still limited data base on genotoxicity test results with nanomaterials and provide congruent results of a battery of standard OECD test methods applied to nanomaterials.

yearjournalcountryeditionlanguage
2010-10-06Nanotoxicology
https://doi.org/10.3109/17435390.2010.506694