Halloysite nanotubes-carbon dots hybrids multifunctional nanocarrier with positive cell target ability as a potential non-viral vector for oral gene therapy

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RESEARCH PRODUCT

Halloysite nanotubes-carbon dots hybrids multifunctional nanocarrier with positive cell target ability as a potential non-viral vector for oral gene therapy

Marina Massaro Giuseppa Biddeci Serena Riela Giuseppe Nicotra Corrado Spinella Giampaolo Barone G. Spinelli Francesco Di Blasi Giuseppe Cavallaro Giuseppe Lazzara

subject

Circular dichroism Cell Survival Surface Properties Static Electricity Administration Oral 02 engineering and technology Cellular imaging engineering.material 010402 general chemistry 01 natural sciences Halloysite Antioxidants Biomaterials HeLa Colloid and Surface Chemistry Dynamic light scattering Fluorescence microscope Tumor Cells Cultured Carbon dots Animals Humans Particle Size Settore CHIM/02 - Chimica Fisica Drug Carriers biology Molecular Structure Halloysite nanotubes Chemistry Nanotubes Carbon Optical Imaging Gene Transfer Techniques Transfection DNA Settore CHIM/06 - Chimica Organica 021001 nanoscience & nanotechnology biology.organism_classification Dark field microscopy DNA interaction 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Halloysite nanotubes Carbon dots DNA interaction Cellular imaging engineering Biophysics Cattle Nanocarriers 0210 nano-technology Porosity HeLa Cells

description

Abstract Hypothesis The use of non-viral vectors for gene therapy is hindered by their lower transfection efficiency and their lacking of self-track ability. Experiments This study aims to investigate the biological properties of halloysite nanotubes-carbon dots hybrid and its potential use as non-viral vector for oral gene therapy. The morphology and the chemical composition of the halloysite hybrid were investigated by means of high angle annular dark field scanning TEM and electron energy loss spectroscopy techniques, respectively. The cytotoxicity and the antioxidant activity were investigated by standard methods (MTS, DPPH and H2O2, respectively) using human cervical cancer HeLa cells as model. Studies of cellular uptake were carried out by fluorescence microscopy. Finally, we investigated the loading and release ability of the hybrid versus calf thymus DNA by fluorescence microscopy, circular dichroism, dynamic light scattering and ζ-potential measurements. Findings All investigations performed confirmed the existence of strong electrostatic interactions between the DNA and the halloysite hybrid, so it shows promise as a multi-functional cationic non-viral vector that has also possesses intracellular tracking capability and promising in vitro antioxidant potential.

year	journal	country	edition	language
2019-01-01

10.1016/j.jcis.2019.05.062 http://hdl.handle.net/10447/368359