6533b861fe1ef96bd12c5a7c

RESEARCH PRODUCT

SAXS Study on Myoglobin Embedded in Amorphous Saccharide Matrices

Massimo PanzicaAlessandro LongoSergio GiuffridaFm Giordano

subject

Small-angle X-ray scatteringMyoglobinBiophysicsAnalytical chemistrySurfaces and InterfacesGeneral ChemistryMaltoseDisaccharidesTrehaloseSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)Sodium dithionitechemistry.chemical_compoundchemistryMyoglobinX-Ray Diffractionmyoglobin saccharide sugar trehalose sucrose maltose lactose bioprotection biopreservation SAXS Small-Angle X-Ray ScatteringScattering Small AngleAnimalsGeneral Materials ScienceSoft matterLactoseSugarMetmyoglobinBiotechnology

description

We report on Small Angle X-ray Scattering (SAXS) measurements performed on samples of carboxy-myoglobin and met-myoglobin embedded in low hydrated matrices of four different saccharides (trehalose, sucrose, maltose and lactose). Results confirm the already reported occurrence of inhomogeneities, which are not peculiar of trehalose samples, but appear also in maltose and lactose, and in some cases also sucrose, being dependent on the sample hydration and on the presence of sodium dithionite. This behaviour confirms our previous interpretation about the nature of the inhomogeneities, and prompt it as a possible general behaviour for highly concentrated sugar matrices.

10.1140/epje/i2011-11087-6http://hdl.handle.net/10447/60050