6533b862fe1ef96bd12c621d
RESEARCH PRODUCT
Electroporation by concentric-type needle electrodes and arrays.
Keng Li LanAlexey LihachevSaulius ŠAtkauskasWen-shiang ChenYi Kungsubject
0301 basic medicineComputer scienceBiophysicsGene transferGene deliveryConcentric03 medical and health sciencesMice0302 clinical medicineTissue damageElectrochemistryGenomic medicineAnimalsPhysical and Theoretical ChemistryElectrodesZebrafishbusiness.industryElectroporationGene Transfer TechniquesGeneral MedicineBiotechnology030104 developmental biologyElectroporationNeedles030220 oncology & carcinogenesisElectrodebusinessBiomedical engineeringdescription
Abstract The efficacy of genomic medicine depends on gene transfer efficiency. In this area, electroporation has been found to be a highly promising method for physical gene transfer. However, electroporation raises issues related to electrical safety, tissue damage, and the number of required wounds. Concentric-type needle electrodes seek to address these issues by using a lower bias (10 V), a single wound, fewer processing steps, and a smaller working area (≈ 10 mm 3 ), thus offering greater accuracy and precision. Moreover, the needle can be arrayed to simultaneously treat several target regions. This paper proposes a novel method using concentric-type needle electrodes to improve the efficacy of genomic medicine in terms of electrical safety, human factor and usability engineering.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-03-02 | Bioelectrochemistry (Amsterdam, Netherlands) |