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RESEARCH PRODUCT

Multiplex Detection of Aspergillus Species

Rosa AznarRosa AznarMaría V. SelmaP.v. Martínez-culebrasP.v. Martínez-culebras

subject

0301 basic medicineOchratoxin AChromatographybiologyfungi030106 microbiologyAspergillus nigerfood and beveragesbiology.organism_classificationDNA extractionlaw.invention03 medical and health scienceschemistry.chemical_compound030104 developmental biologyReal-time polymerase chain reactionchemistrylawPolyketide synthaseMultiplex polymerase chain reactionbiology.proteinMultiplexPolymerase chain reaction

description

Multiplex real-time polymerase chain reaction (PCR) provides a fast and accurate DNA-based tool for the simultaneous amplification of more than one target sequence in a single reaction. Here a duplex real-time PCR assay is described for the simultaneous detection of Aspergillus carbonarius and members of the Aspergillus niger aggregate, which are the main responsible species for ochratoxin A (OTA) contamination in grapes. This single tube reaction targets the beta-ketosynthase and the acyl transferase domains of the polyketide synthase of A. carbonarius and the A. niger aggregate, respectively.Besides, a rapid and efficient fungi DNA extraction procedure is described suitable to be applied in wine grapes. It includes a pulsifier equipment to remove conidia from grapes which prevents releasing of PCR inhibitors.

yearjournalcountryeditionlanguage
2016-12-07
https://doi.org/10.1007/978-1-4939-6707-0_17