6533b862fe1ef96bd12c6d3a
RESEARCH PRODUCT
Antispasmodic effect of 4'-methylepigallocatechin on guinea pig ileum.
Rosilene M. MarçalCosimo PizzaFábia Valéria Menezes SouzaMarcelly Barbosa Da RochaAntônio Euzébio Goulart SantanaCharlez Dos Santos Estevamsubject
AntispasmodicBarium CompoundsGuinea Pigs4′-MethylepigallocatechinIleumPharmacologyCatechinPotassium ChlorideGuinea pigCalcium ChlorideChloridesIleum4'-methylepigallocatechinDrug DiscoverymedicineAnimalsGuinea pig ileumPharmacologyChemistryPlant ExtractsGuinea pig ileumParasympatholyticsMuscle SmoothGeneral MedicineMaytenusSymptomatic reliefIn vitromedicine.anatomical_structureVerapamilAnesthesiaAntispasmodicAntispasmodic EffectCarbacholmedicine.drugHistamineMuscle Contractiondescription
AbstractThe antispasmodic effect of 4′-methylepigallocatechin (MEC), which was isolated from Maytenus rigida Mart (Celestraceae), was investigated in vitro in guinea pig intestinal segments. In the isolated ileum, MEC (1 nM–100μM) did not modify the ileal spontaneous tonus or the electrically elicited contractions. MEC (8μM) significantly (p<0.01) reduced the submaximal contractions induced by histamine (2μM), carbachol (100μM) and BaCl2 (0.03M). An additive relaxing action (p<0.001) was observed by co-incubation of verapamil (10 nM) and MEC (8μM). Although MEC (1nM–100μM) did not modify the contractions elicited by 60mM KCl, it significantly reduced the CaCl2 contractile response without changing the EC50 (effective concentration of CaCl2 causing 50% of maximum response). In brief, these results show that MEC has a potent ileal spasmolytic effect and blocks spasms induced by specific and nonspecific stimuli. Importantly, the spasmolytic effects were attained at low concentrations and might be related to the symptomatic relief of abdominal pain that is obtained from the use of the M. rigida stem bark.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-10-01 | Fitoterapia |