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RESEARCH PRODUCT

Srebf2 Locus Overexpression Reduces Body Weight, Total Cholesterol and Glucose Levels in Mice Fed with Two Different Diets

Sebastian BlesaSergio Martínez HervasA.b. Garcia-garciaHerminia González-navarroFelipe J. ChavesAntonio Ferrández-izquierdoJosé T. RealJulian CarreteroIrene Andrés-blascoÁNgela Vinué

subject

0301 basic medicinemedicine.medical_specialtymedicine.medical_treatment030209 endocrinology & metabolismlcsh:TX341-641Carbohydrate metabolismtransgenic miceArticle03 medical and health scienceschemistry.chemical_compound0302 clinical medicineInternal medicineAdipocyteDiabetes mellitusHyperlipidemialipid metabolismmedicinecarbohydrate metabolismhigh-sucrose diethigh-fatNutrition and DieteticsCholesterolInsulinType 2 Diabetes MellituscholesterolLipid metabolismmedicine.diseaselipoproteins030104 developmental biologyEndocrinologychemistrylipids (amino acids peptides and proteins)atherosclerosissterol regulatory element-binding protein 2 (SREBP-2)lcsh:Nutrition. Foods and food supplyFood Science

description

Macronutrients represent risk factors for hyperlipidemia or diabetes. Lipid alterations and type 2 diabetes mellitus are global health problems. Overexpression of sterol regulatory element-binding factor (Srebf2) in transgenic animals is linked to elevated cholesterol levels and diabetes development. We investigated the impact of increased Srebf2 locus expression and the effects of control and high-fat, high-sucrose (HFHS) diets on body weight, glucose and lipid metabolisms in transgenic mice (S-mice). Wild type (WT) and S-mice were fed with both diets for 16 weeks. Plasma glucose, insulin and lipids were assessed (n = 25). Immunostainings were performed in liver, pancreas and fat (N = 10). Expression of Ldlr and Hmgcr in liver was performed by RT-PCR (N = 8). Control diet: S-mice showed reduced weight, insulin, total and HDL cholesterol and triglycerides (TG). HFHS diet widened differences in weight, total and HDL cholesterol, insulin and HOMA index but increased TG in S-mice. In S-mice, adipocyte size was lower while HFHS diet produced lower increase, pancreatic &beta

10.3390/nu12103130https://www.mdpi.com/2072-6643/12/10/3130