6533b862fe1ef96bd12c75ed

RESEARCH PRODUCT

Zymography Methods to Simultaneously Analyze Superoxide Dismutase and Catalase Activities: Novel Application for Yeast Species Identification

Esther Gamero-sandemetrioRocío Gómez-pastorRocío Gómez-pastorEmilia MatallanaEmilia Matallana

subject

0301 basic medicineGene isoformchemistry.chemical_classificationbiologySOD2Molecular biologyYeastSuperoxide dismutase03 medical and health sciences030104 developmental biology0302 clinical medicineEnzymechemistryCatalasebiology.proteinZymographyPolyacrylamide gel electrophoresis030217 neurology & neurosurgery

description

We provide an optimized protocol for a double staining technique to analyze superoxide dismutase enzymatic isoforms Cu-Zn SOD (Sod1) and Mn-SOD (Sod2) and catalase in the same polyacrylamide gel. The use of NaCN, which specifically inhibits yeast Sod1 isoform, allows the analysis of Sod2 isoform while the use of H2O2 allows the analysis of catalase. The identification of a different zymography profiling of SOD and catalase isoforms in different yeast species allowed us to propose this technique as a novel yeast identification and classification strategy.

yearjournalcountryeditionlanguage
2017-01-01
https://doi.org/10.1007/978-1-4939-7111-4_17